Study of the use of oxidation enzymes and microbial hydrolysis in cleaning antique linen textiles

Document Type : Original Article

Authors

1 Faculty of Archeology, Sohag University

2 National Research Center

3 Faculty of Archeology, Cairo University

Abstract

Spots and dirt are considered one of the most important 
destructive factors for antique textiles, and the process of 
searching for new techniques for treatment and maintenance is 
also a necessity to get rid of such stains and dirt. The study used 
one of the oxidation enzymes "Laccase enzyme isolated from the 
genus Alternaria tenuissima KM651985 in cleaning fungal stains 
on archaeological linen textiles, as well as using basal protease 
enzymes isolated from bacterial strains Bacillus lichiniformis 
P12 and Bacillus pumilus p19 in cleaning protein stains "blood 
stains". 
On the surfaces of linen textiles, the study relied, in the process 
of evaluating the effectiveness of the cleaning process with 
enzymes, on measuring the chromatic change values of the 
samples ΔE before and after the treatment process, as well as 
measuring the optical density of the washing solutions resulting 
from the treatment process using a spectro photometer. Fungal 
stains as well as enzyme efficiency The study concluded that the 
efficiency of the protease enzyme isolated from the genus 
Bacillus lichiniformis P12 was higher than the efficiency of the 
protease enzyme isolated from the genus Bacillus pumilus p19 in 
removing protein stains from the surfaces of linen textiles 

Keywords